Fig. 5

NMR probe targeting hSHMT. a Schematic illustration of hSHMT NMR probe 2. b ¹⁹F NMR spectral change of probe 2 (5 mM) upon the addition of hSHMT1 (5 units/mL). Assay conditions: 5 units/mL hSHMT1, 50 mM HEPES buffer (pH 7.5), 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 30% D₂O, with or without inhibitor (±)-SHIN1 10 µM, 0.1% DMSO, 37 °C. CF₃COOH (–76.5 ppm) was used as the internal standard for ¹⁹F NMR. c Conversion rate of hSHMT probe 2 by hSHMT1 with (blue square) or without (red circle) hSHMT inhibitor (±)-SHIN1 10 µM. Error bars represent s.d., n = 3. d ¹⁹F NMR spectra of probe 2 (1 mM) in rat liver homogenate (2.42 mg proteins/mL in PBS) with or without inhibitor (±)-SHIN1 10 µM. e ¹H (T₂-weighted) and ¹⁹F chemical-shift-selective imaging (11.7 T) of probe 2 (10 mM). Assay conditions: hSHMT1 (1 unit/mL), with or without inhibitor (±)-SHIN1 10 µM, 37 °C, acquisition time = 17 min. Source data of Fig. 5c are provided as a Source Data file