Fig. 1

Study overview. Four analysis steps were used to identify transcriptomic markers of risk of HIV-1 acquisition among RV144 vaccinees. A first transcriptomic dataset of blood collected from 40 HIV-1 negative vaccinees and 10 HIV-1 negative placebo recipients prevaccination and 2 weeks after vaccination was used to identify pathways modulated by the RV144 vaccine (step 1). A second independent transcriptomic dataset of blood collected from 183 case–control vaccinees (including 31 infected participants) and 30 placebos (including 17 infected participants), 2 weeks after vaccination was used to identify pathways associated with HIV-1 acquisition. Logistic regression was used to build a multi-OMICS classifier of HIV-1 acquisition among RV144 vaccinees (step 3) and a projection-based integrative analysis was used to associate the different OMICS to identify mechanistic mediators of vaccine response (step 4). Three elements (“syringe [https://www.svgrepo.com/svg/10894/injecting-syringe]”, “blood tube [https://www.svgrepo.com/svg/33576/blood-test]” and “man [https://www.svgrepo.com/svg/3680/standing-frontal-man-silhouette]”) were modified and used in the figure under “CC-BY 4.0 [https://creativecommons.org/licenses/by/4.0/]” license