Fig. 2
Age-dependent NM accumulation in AAV-hTyr-injected rats. a Hematoxylin-eosin (H&E)-stained brain sections showing progressive intracellular NM accumulation (brown) within ipsilateral SNpc DA neurons from AAV-hTyr-injected rats. Scale bar, 12.5 μm. b Quantification of intracellular NM optical density in ipsilateral SNpc DA neurons of AAV-hTyr-injected rats. *p < 0.05, compared to 0.5 m; #p < 0.05, compared to 1 m; §p < 0.05, compared to 2&4 m. c Left, quantification of intracellular NM optical density in post-mortem SNpc sections from elderly human (average 80 years old) control subjects, age-matched ILBD subjects and age-matched idiopathic PD patients. *p < 0.05, compared to control subjects. Right, H&E-stained human brain sections. Scale bar, 12.5 μm. In b, c values are mean ± SEM. In b, n = 163 neurons from n = 7 rats (0.5 m), n = 136 neurons from n = 5 rats (1 m), n = 174 neurons from n = 5 rats (2 m), n = 162 neurons from n = 7 rats (4 m), n = 172 neurons from n = 6 rats (12 m), n = 179 neurons from n = 6 rats (24 m). In c, n = 1436 neurons from n = 6 control subjects, n = 640 neurons from n = 3 ILBD subjects, n = 644 neurons from n = 10 PD subjects. See Supplementary Table 1 for additional information on the human subjects used for the analyses in c. Statistical analyses: ANOVA on ranks; Dunn’s post-hoc test. Photomicrographs correspond to 5-μm-thick sections
