Fig. 3
STM downregulation is important for flower initiation. a, b Primordium initiation defect of mp-S319 compared to mp-S319 without (Mock) and with conditional increase in STM activity upon activation of p35S:STM-GR with the synthetic steroid dexamethasone (Dex). a Phenotype; white arrow points to developing flowers. Scale bar = 1 mm. b Quantification of flowers initiated. Box and whisker plot: Lower vertical bar = sample minimum. Lower box = lower quartile. Black Line = median. Upper box = upper quartile. Upper vertical bar = sample maximum. NS P-value = 0.11, *P-value = 0.024 one-tailed Mann–Whitney U test. n = 15 and 11 (WT mock and dex) n = 8 and 5 (mp-S319 mock and dex). Source data are provided as a Source Data file. c, d Primordium initiation defect of mp-S319 ett arf4 compared to mp-S319 ett arf4 plants in which STM expression is knocked down using an artificial microRNA, amiRSTM35. c Phenotype; white arrow points to developing flowers. Scale bar = 1 mm. d Quantification of flowers initiated. Box and whisker plot: Lower vertical bar = sample minimum. Lower box = lower quartile. Black Line = median. Upper box = upper quartile. Upper vertical bar = sample maximum. NS P-value = 0.99; ***P-value = 0.00001 one-tailed Mann–Whitney U test. n = 11(arf4), n = 13 (arf4 p35S:amiRSTM), n = 8 (mp-S319 ett arf4), n = 15 (mp-S319 ett arf4 p35S:amiRSTM). arf4 is indistinguishable from the wild type20. Source data are provided as a Source Data file
