Fig. 5
The MP target FIL promotes flower initiation together with ETT/ARF4. a Flower initiation phenotypes of wild type (WT), ett arf4, fil-8, young and old fil-8 ett arf4 and auxin transport inhibitor (NPA) treated fil ett arf4 plants. NPA (500 nM), was applied from 11 days of age until bolting. Scale bar = 1 mm. b Quantification of lateral organs formed in the absence and presence of NPA. Box and whisker plot: Lower vertical bar = sample minimum. Lower box = lower quartile. Black Line = median. Upper box = upper quartile. Upper vertical bar = sample maximum. P-values ( ± NPA): NS P = 0.11 (WT), NS P = 0.73 (fil/ + ett arf4) and *P = 0.006 (fil ett arf4), one-tailed Mann–Whitney U test. n = 7 (WT mock) n = 5 (WT NPA) n = 4 (fil/ + ett arf4 mock), n = 4 (fil/ + ett arf4 NPA) n = 5 (fil ett arf4 Mock) n = 5 (fil ett arf4 NPA). See supplementary Fig. 7a for images of mock and NPA treated control plants. Source data are provided as a Source Data file. c Relative expression of STM, IPT7 and FIL normalized over that of UBQ10 in the genotypes indicated. Shown are mean ± SEM of one representative of three experiments. Source data are provided as a Source Data file. d Chromatin immunoprecipitation (ChIP) to test FIL binding to conserved regions of the STM locus. Top: STM locus; green triangles, conserved FIL binding sites (AATNATAA)41. TA3 served as negative control. ChIP was performed under identical conditions in the WT. Shown are mean ± SEM of three experiments. Source data are provided as a Source Data file
