Fig. 7
STM repression by FIL and ETT/ARF4 relies on reduced histone H3 acetylation. a, b Flower initiation phenotypes of wild type (WT), hda19, the hypomorph mp-S319 mutant and mp-S319 hda19. a Phenotype; Scale bar = 1 mm. b Quantification of flowers formed; ***P = 0.00024, one-tailed Mann–Whitney U test. n = 11 (WT) n = 11 (mp-S319) n = 9 (mp-S319 hda19). c Relative expression of STM and IPT7 in trimmed inflorescences normalized over that of UBQ10. Shown are mean ± SEM of three experiments. d Chromatin immunoprecipitation (ChIP) to test HDA19 binding to conserved regions of the STM locus in inflorescences. TA3 served as negative control. ChIP was performed under identical conditions in the WT. Shown are mean ± SEM of three experiments. e Anti-histone 3 lysine 27 acetylation (H3K27ac) compared to H3 ChIP at the STM locus in expanded leaves of WT, fil/+ ett arf4 and fil ett arf4. TA3 served as negative control. ChIP was performed under identical conditions in the WT. Shown are mean ± SEM of three experiments. b–e Source data are provided as a Source Data file. f Model for the repression of STM in incipient reproductive primordia. See text for details
