Fig. 5
Comparison of Amino acid usage in TCR CDR3 FG-loop from MHCr, MHCi and pre-selection repertoires. a, b Pearson correlations calculated using normalized amino acid usage among MHCi, MHCr and pre-selection TCR repertoires. The FGβ- (a) and FGα-loop (b) amino acid usage frequencies of individual repertoires are normalized against those of B6 DP_1 and MHCKO DP, respectively. The pair-wise Pearson correlation coefficients were displayed as heatmaps to indicate the distinct amino acid usages of MHCi, MHCr and pre-selection TCR repertoires. c, d The frequency of Cys-containing TCRβ (c) and TCRα (d) sequences in MHCr, MHCi and the pre-selection repertoires. e, f Fold changes of charged and hydrophobic amino acid usages observed in various repertoires. Amino acid frequencies are normalized to those in B6_DP1 repertoire
