Fig. 4
From: Synthetic energy sensor AMPfret deciphers adenylate-dependent AMPK activation mechanism
Effects of adenylate mixtures on FRET changes. a Free ATP, not Mg2+-complexed ATP, competes with AMP. MgCl2 does not affect AMPfret 1.0 baseline FRET (blue bars) and FRET induced by AMP (orange) or ADP (yellow), but prevents ATP-induced FRET inhibition (red). Plain and dashed bars, respectively, correspond to FRET signal measured in absence or in presence of Mg2+, respectively. b AMPfret 2.1 responses to individual adenylates and adenylate mixtures as indicated below the bars: no adenylates (blue), AMP (orange), ADP (yellow), ATP (red) AMP plus ADP (orange dashed yellow), mixtures of AMP, ADP, and ATP (gray). Bars to the right represent physiological mixtures with a shift to energy deprivation and emphasize the role of free ATP. The FRET signals are normalized to control (no adenylates). All data and error bars represent mean ± SEM (n ≥ 3). After checking normality and equality of variance, statistical significance was analysed by one-way ANOVA followed by Bonferroni multiple comparison. Means sharing the same letter do not differ significantly (p ≤ 0.02)
