Fig. 2
From: Barcode-free next-generation sequencing error validation for ultra-rare variant detection
NGS error verification of sequence-known DNA sample. a Miscalled base errors validation through a known-sequence template sequencing. Out of total variants (1889) called, 99.58% (1879) was miscalled bases, and 0.15% (10) was true variants. b Comparison of variants counts before and after the barcode-free NGS error validation according to error type. c True variants expected by the barcode-free NGS error validation method; the 10 variants (0.53% of the variant call) were in both of 454 and validation sequencing result that the DNA damage caused. Source data are provided as a Source Data file
