Fig. 1

Coculture with breast cancer cells increases miR-375 in human MΦ. a–l Human PBMC-derived MΦ were used. a Heatmap of differentially expressed miRs from control and MΦ cocultured with MCF-7 cells (n = 4). b Representative differentially expressed miRs in control MΦ vs. cocultured MΦ and c MA-plot. d MiR-375 abundance was measured in control, 48 h cocultured, polarized (LPS + IFNγ for 24 h, IL-4 for 48 h) and resolution-phase (resolvin D1 for 6 h) MΦ via qPCR, and normalized to untreated MΦ (n ≥ 3). e Primary human MΦ were treated for 3 h with actinomycin D (Act D) or a DMSO control. Cells were washed and cocultured with MCF-7 cells for 24 h. MiR-375 abundance was quantified via qPCR and normalized to MΦ control (n = 8). f PPARgamma mRNA expression was measured as a positive control (n ≥ 5). g–j MΦ were transfected with nonspecific control (ns siRNA) or DICER siRNA for 24 h and cocultured with MCF-7 cells for another 48 h (n = 5–6). g DICER mRNA expression in MΦ. h Endogenous miR-21-5p and miR-142-3p were measured by qPCR as a control. i MiR-375 abundance and j pre-miR-375 expression were measured by qPCR in control and cocultured MΦ. k MΦ were cocultured with indicated cell lines for 24 h. MiR-375 levels were measured by qPCR and normalized to MΦ control (n ≥ 5). l MΦ were cocultured with MCF-7 control (empty vector transfected) or decoy (miR-375 decoy transfected) cells for 24 h. MiR-375 expression was measured by qPCR and normalized to MΦ control (n = 27) using different MCF-7 cell passages. Data of b and d–I are mean ± SEM and p-values were calculated using two-tailed Student’s t-test (d, f, g–k) and one-sample t-test (e, l). *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant