Fig. 9
miR-375 in human invasive breast carcinoma. Human invasive mammary carcinoma tissue microarrays sections were analyzed for miR-375 abundance and CCL2 mRNA expression by in situ hybridization (light blue), followed by staining of nuclei with DAPI (white) and MERTK protein (blue). Bright-field signal of miR-375 was converted to fluorescence image using Inform 2.4.0 and ImageJ software, to present colocalization of miR-375, MERTK, and CCL2 from two consecutive sections. a Mean signal intensity of miR-375 in human invasive breast cancer (breast cancer) sections compared with normal breast tissue sections is shown (n = 156 breast tumors; n = 49 normal breasts). b Mean intensity of miR-375 in human ductal carcinoma in situ (DCIS) sections compared with normal breast tissue sections is shown (n = 16 DCIS tumors; n = 49 normal breasts). c Correlation between miR-375 mean intensity and MERTK expression in invasive breast tumor sections (n = 155). d Representative pictures of invasive breast cancer section and normal breast with arrowheads in magnification showing miR-375 (red) colocalization with MERTK (blue). e Mean intensity of miR-375 in ER + and ER − invasive breast tumor sections (n = 96 ER + and n = 43 ER − sections). f Mean miR-375 intensity of HER2 + and HER2 − invasive breast tumor sections (n = 16 HER2 + and n = 89 HER2 − sections). g Correlation between miR-375 mean intensity and CCL2 mRNA expression in invasive breast tumor sections (n = 155). h Representative pictures of invasive breast cancer section and normal breast with arrowheads in magnification showing miR-375 (red) colocalization with MERTK (blue) and CCL2 (light blue). Data are mean ± SEM and p-values were calculated using two-tailed Student’s t-test. *p < 0.05, ***p < 0.001, n.s., not significant
