Fig. 7
From: Primary tumor-induced immunity eradicates disseminated tumor cells in syngeneic mouse model
DTCs are eliminated after the complete resection of primary EMT6 tumors. a Time line of experimental procedures. b EMT6-Luc cells were first implanted orthotopically into the mammary fat pads of BALB/c mice (n = 5–10 for each group), primary tumors were resected 3 weeks post implantation, and the anti-CD8 antibody treatment was started 60-day post resection. Animals were then monitored by weekly bioluminescence imaging up to 150 days and no signal was detected in both control- and antibody-treated groups. c, d CD8+ T-cell depletion in treated animals was monitored weekly for 4 weeks post treatment and a significant depletion of CD8+ T cells was demonstrated by analyzing the PBMCs. e Mice were killed and pan-keratin staining was performed in the lung sections of treated animals showing no detectable pan-keratin positive cells. Scale bar: 50 µm. Results are presented as mean ± SD (n = 3). ***P < 0.0005, two-way analysis of variance test
