Table 1 Cryo-EM data collection and image processing
Microscope | Titan Krios (Thermo Fisher Scientific) |
Camera | K2 Summit (Gatan) |
Acceleration voltage (kV) | 300 |
Magnification | 130,000 |
Defocus range (μm) | 0.4–4.6 |
Dose rate (e− pixel−1 s−1) | 5.78 |
Number of movie frames | 30 |
Exposure time (s) | 6 |
Total electron dose (e− Å−2) | 32 |
Pixel size (Å) | 1.041 |
Gatan imaging filter | 20 eV |
Mode | Counting mode |
Box size (pixel) | 320 |
Inter box distance (Å) | 28.8 |
Number of extracted segments | 119,395 |
Number of segments after 2D classification | 62,250 |
Number of segments after 3D classification | 32,677 |
Resolution, 0.143 FSC criterion (Å) | 3.3 |
Map sharpening B-Factor (Å2) | 119.6 |
Helical rise (Å) | 4.8 |
Helical twist (°) | 0.58 |
Symmetry imposed | C1 |
