Fig. 1
From: The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells
CRISPR-ON ES cells for Dox-inducible activation of endogenous Nanog. a Schematic representation of the Nanog locus (black arrow: promoter; yellow boxes: exons; grey box: Nanog enhancer; red arrowhead: gRNA). Below, the position of the amplicons and probe used for the assays indicated on the right is shown. b ChIP across the Nanog locus monitoring total histone H3 (top) and pan-acetyl H3 (bottom) in the absence (blue) and after 72 h of Dox treatment (red). Each dot represents normalised %IP measured in individual replicates and lines the averages. c Normalised levels of Nanog mRNA (top) and pre-mRNA (bottom) after the indicated number of days in the absence (blue) and the presence (red) of Dox. Each dot represents measurements in individual replicates as in b. d Representative smFISH image using intronic Nanog probes before and after 72 h of Dox induction. e Normalised levels of eRNA production from the −5 kb enhancer presented as in c. In all panels, n = 4; 2 with each independent SunTag clone
