Fig. 1
From: Dietary fatty acids fine-tune Piezo1 mechanical response
Margaric acid inhibits Piezo1 currents in N2A cells. a Representative whole-cell patch-clamp recordings (at −60 mV) of control and margaric acid (MA) (1, 10, 25, 50, 100, and 300 µM)-treated N2A cells elicited by mechanical stimulation. Bottom right panel displays representative Piezo1 macroscopic currents of MA-treated N2A cells incubated with Yoda1 prior to mechanical stimulation. The structure of MA is displayed on top. b Piezo1 current densities elicited by maximum displacement of MA-treated N2A cells. A Boltzmann function, Eq. (2), was fitted to the data (IC50 = 28.3 ± 3.4 SEM). Circles are mean ± SD. n is denoted above the x-axis. c Piezo1 current densities elicited by maximum displacement of control and MA (10 µM; 18 h and 5 days)-treated N2A cells. n is denoted above the x-axis. Kruskal–Wallis and Dunn’s multiple comparisons test. d MA membrane content fold change in N2A cells treated with MA 100 µM for 18 h or 10 µM each day for 5 days, as determined by LC-MS. e Piezo1 current densities elicited by maximum displacement of control, MA (100 µM)-treated N2A cells, and MA (100 µM)-treated N2A cells incubated with 15 µM Yoda1 prior to mechanical stimulation. Bars are mean ± SD. n is denoted above the x-axis. Kruskal–Wallis and Dunn’s multiple comparisons test. f Boxplots show the mean, median, and the 75th to 25th percentiles of the displacement thresholds required to elicit Piezo1 currents of control and MA-treated N2A cells. n is denoted above the x-axis. One-way ANOVA and Bonferroni test. g Current changes elicited by maximum displacement of N2A cells perfused for 120 s with bath solution (control), MA (100 μM), and Gd3+ (30 μM) consecutively. Gd3+ was used as control for the perfusion. Data samples are paired. n is denoted above the plot. Friedman test. Asterisks indicate values significantly different from control (∗∗∗p < 0.001 and ∗∗p < 0.01) and n.s. indicates not significantly different from the control
