Fig. 7
From: Dietary fatty acids fine-tune Piezo1 mechanical response
EPA supplementation abrogates the phenotype of Piezo1 xerocytosis mutations. a Ribbon representation of Mus musculus (mm) Piezo1 monomer (PDB ID: 5Z10) highlighting equivalent residues that when mutated cause dehydrated hereditary stomatocytosis in humans. b Representative normalized macroscopic currents (at −60 mV) evoked by maximum displacement of N2A cells transfected with human Piezo1 dehydrated hereditary stomatocytosis mutants R1943Q, M2225R, R2302H, R2456, and R2488Q with and without EPA supplementation (left and right, respectively). Human Piezo1 wild type (WT) without supplementation is shown for comparison. c Piezo1 time constants of inactivation elicited by maximum displacement of dehydrated hereditary stomatocytosis mutants R1943Q, M2225R, R2302H, R2456H, and R2488Q with and without EPA supplementation. Human Piezo1 WT without supplementation is shown for comparison. Bars are mean ± SD. Unpaired t-test with Welch correction, except for R2302H in which Mann–Whitney test was used. d Boxplots show the mean, median, and the 75th to 25th percentiles of the displacement threshold required to elicit currents of Piezo1 dehydrated hereditary stomatocytosis mutants R1943Q, M2225R, R2302H, R2456H, and R2488Q with and without EPA supplementation. Human Piezo1 WT without supplementation is shown for comparison. Unpaired t-test, except for R2488Q in which Mann–Whitney test was used. Asterisks indicate values significantly different from control (∗∗∗p < 0.001 and ∗∗p < 0.01) and n.s. indicates not significantly different from the control. n is denoted above the x-axes
