Fig. 4
B lymphocytes in the late phase after ischemia-reperfusion injury in mice. a, b Semi-quantitative evaluation of immune cell infiltrates in the kidney at different time points after IRI or sham surgery as determined by CIBERSORT analysis on whole kidney RNAseq data (n = 3/group, Mann–Whitney test in comparison to sham control, *P < 0.05). c–f Flow cytometric analysis on CD45+CD3−CD19+ or dim leukocytes isolated from the kidney or the bone marrow (BM) 16–18 months after IRI (1 representative example of 6 replicates is shown). General gating strategy is presented in Supplementary Fig. 5. c, d CD138 was absent on renal B cells, but detectable on the bone marrow B cells isolated from the same mouse (additional controls presented in Supplementary Fig. 3). e, f CD45+CD3−CD19+ or dimCD45R− B cells expressed high levels of CD126 and CD184 (Cxcr4). g, h RPKM values from RNAseq analysis on whole kidney 12 months after IRI or in sham controls focused on immunoglobulin constant regions transcripts (n = 3/group, Sidak’s multiple comparison test, *adjusted-P < 0.05, ***adjusted-P < 0.001). In all histograms, mean values and SE are shown
