Fig. 5
B cell receptor analysis and detection of autoantibodies. a, c Absolute RPKM values for germline Ighv and Igkv transcripts in 3 replicates collected 6 and 12 months after IRI and from sham control (matched to the 12 month IRI samples). The most frequent genes are highlighted with colors as indicated (mean values and SE are shown; n = 3/group; Mann–Whitney test, ****P < 0.0001 in comparison to sham control). b, d Relative frequency of germline Ighv and Igkv transcripts over time indicating the enrichment of common genes in the late phase; the number indicates the total RPKM of Ighv and Igkv at each time point. e, f Immunoseq analysis showing representatives cluster of vj rearrangements in sham and IRI mice (both 12 months after surgery). Each circle represents a unique amino acid sequence. The sequence is reported in the figure if >2 rearrangements were detected. The number of rearrangements is indicated below the amino acid sequence. The lines connect the circles if the corresponding rearrangements differ only in 1 amino acid. g Volcano plot comparison of signal-to-noise ratio in IgG reactivity in plasma samples collected 16–18 months after IRI (n = 6) and in age-matched controls (n = 6) on a microarray of autoantigens, with indication of log2-fold change on the x-axis (>0 indicates higher in IRI) and significance level, expressed as log10 of FDR-adjusted P-value. The name of representative antigens is indicated
