Fig. 2

UFL1 regulates the ATM signaling. a U2OS cells expressing UFL1 tet-on shRNA1 were irradiated with 2 Gy IR. Thirty minutes later, cells were lysed and blotted with indicated antibodies. b Representative picture of γH2AX, 53BP1, and BRCA1 foci in control (Dox-) and UFL1 knockdown (Dox+) U2OS cells 1 h after 0.5 Gy treatment. Scale bars, 10 µm. c–e Quantification of intensities of γH2AX, 53BP1, and BRCA1 foci in control (Dox-) and UFL1 knockdown (Dox+) U2OS cells. Data presented as mean ± SD of n = 50 cells. **p < 0.01 by Student’s t-test. Source data are provided as a Source Data file