Fig. 2
Validation of the Crh-Cre rat. a Cre-dependent eYFP expression in the CeA. Scale bar = 200 μm. b Rendered isosurface analysis demonstrated the colocalization of CRF immunoreactivity within CeA neurons that also expressed Cre-dependent eYFP. The arrow points to an example of eYFP and CRF in the same neuron. c Infrared (Left) and eYFP fluorescence (Middle) images of a CeA neuron. (Right) Current clamp recording of the same neuron (manually depolarized to −50 mV), showing hyperpolarization and the suppression of firing in response to the delivery of a 6.5 s train (asterisks) of green light (HaloR, 532 nm). d Representative images of a non-fluorescent CeA neuron and accompanying recording (manually depolarized to −52 mV) and a recording of that neuron that shows no response to light stimulation
