Fig. 2

Nuclear lamina disruption leads to the increased H3 pan-acetylation and transcriptional upregulation in LADs. a Gene expression in Lam-KD and control S2 cells according to the RNA-seq data. Genes located in LADs and inter-LADs are designated by light-blue and light-grey circles, respectively. Differentially expressed (DE) genes, located in LADs and inter-LADs, are designated by black and red rectangles, respectively. b Changes of total transcription according to the RNA-seq data between Lam-KD and control S2 cells in LADs and in inter-LADs. c A representative screenshot from UCSC Genome Browser showing the Lamin–DamID profile in Kc167 cells⁵ and log₂(FC) profiles of H3 pan-acetylation (according to ChIP-seq) and transcription (according to RNA-seq) in Lam-KD/control S2 cells. Chromatin annotation in S2 cells⁴⁰, LAD annotation in Kc167 cells²⁸, and RefSeq gene positions are shown below. d Changes in the transcription level in Lam-KD compared to control S2 cells by RT-PCR analysis for the randomly chosen genes from different LADs (top panel), and for all the genes from the 60D LAD (bottom panel). In the bottom panel, genes SerT, CG3419 and CG4589 are located outside the LAD. Expression data of Crtp, Yu, Ssl, Pros28.1B and CG13581 genes upon Lam-KD in S2 cells are from ref. ¹¹. Error bars show SEM between two independent biological replicates. e Changes of H3 pan-acetylation according to ChIP-seq data between Lam-KD and control S2 cells in LADs and in inter-LADs. In panels (b, e), ****P < 0.0001, ***P < 0.001 in a Wilcoxon test. See Fig. 1g legend for description of boxplot elements