Fig. 4
From: Nuclear lamina integrity is required for proper spatial organization of chromatin in Drosophila
NL disruption partially impairs spatial segregation of active and inactive chromatin. a A representative screenshot from the UCSC Genome Browser showing correspondence between the positive PC1 values and the transcriptionally active genome regions. Box plots show transcription level in 20-kb genomic bins possessing positive (n = 4073) and negative (n = 1868) PC1 values. **** – P < 0.0001 in a Wilcoxon test. b Heatmap showing log2 values of contact enrichment for the intra-chromosomal contacts in the control cells between genomic regions as a function of their PC1 values (saddle plot). c Subtraction of saddle plots in Lam-KD cells from that in control cells. d Changes of averaged contact frequency between and within active and inactive fractions of chromatin in Lam-KD compared to control cells. ****P < 0.0001, ***P < 0.001 in a Wilcoxon test. See Fig. 1g legend for description of boxplot elements represented on panels (b) and (d)
