Fig. 5
From: Nuclear lamina integrity is required for proper spatial organization of chromatin in Drosophila
Chromatin density is decreased in a TAD following its detachment from the NE. a Genomic region 36C carrying probes for two-colour FISH analysis. The Hi-C map from untreated S2 cells, lamin–DamID profile from Kc167 cells5, the occupancy of 9 chromatin types from S2 cells40, LAD annotation from Kc167 cells28, and RefSeq gene positions are shown. b Radial-normalised distance (RND) between two FISH probes in Lam-KD (n = 175) and control (n = 175) S2 cells. *P < 0.05 in a Kolmogorov-Smirnov one-sided test. c RND between two FISH probes in the untreated S2 cells. RND < 0.15, n = 84, RND > 0.15, n = 124. **P < 0.01 in a Kolmogorov-Smirnov one-sided test. A representative example of two-colour FISH signals (red and green) in a nucleus stained with anti-LBR antibody is shown below. Nuclear shell of 0.15 R width (R nuclear radius) where the signals are in visible contact with the NL is outlined by the dotted line. Scale bar 1 µm. See Fig. 1g legend for description of boxplot elements represented on panels (b, c)
