Fig. 6
From: Rad52 prevents excessive replication fork reversal and protects from nascent strand degradation
RAD52 affects fork recruitment of SMARCAL1 and RAD51. a Analysis of SMARCAL1 fork recruitment by in situ EdU-proximity ligation assay (PLA). Cells were treated as indicated. The graph shows the number of PLA spots per cell. As a negative control for the PLA, cells were incubated with only the anti-biotin antibody (EdU only). Values are presented as means ± SE (ns not significant; ***P < 0.001; ****P < 0.0001; Mann–Whitney test; N = 145). Representative images are shown. b Analysis of nascent and parental iododeoxyuridine (IdU)-RAD51 PLA. Cells were treated as in the scheme. The graph shows the number of PLA spots per cell ± SE. As a negative control for the PLA, cells were incubated with only the anti-IdU antibody (IdU only). Values are presented as means ± SE (**P < 0.01; ****P < 0.0001; Mann–Whitney test. Nascent DNA N = 170; Parental ssDNA N = 379). Representative images are shown. c Analysis of nascent ssDNA formation in RAD51 overexpressing cells. On top, western blot analysis showing RAD51 overexpression and the experimental scheme used. The graph shows the intensity of ssDNA staining in at least 100 nuclei from two independent experiments. Values are presented as means ± SE (ns not significant; ***P < 0.001; ****P < 0.0001; Mann–Whitney test; N = 436). Representative images are shown. Scale bar represents 5 µm. Source data are provided as a Source Data file
