Fig. 4
From: NKG2A is a NK cell exhaustion checkpoint for HCV persistence
NK cells instructed T cells in HCV persistence. a Mice were treated as in Fig. 3d. ELISpot assays using splenocytes and the indicated epitope peptides. C/OTg mice were i.p. pre-treated with mock IgG (n = 9) or b anti-AGM1 (20 μg/3 days, n = 9), c anti-CD8 (100 μg/3 days, n = 6), together with anti-NKG2A antibody 1 d before HCV inoculation. b HCV-specific CD8+ T cell response by IFN-γ ELISpot and c hepatic and serum HCV RNA copies were measured 2 wpi. Dash lines indicated limits of detection of related assays (qPCR, 500 copies/mL serum or 100 copies/mg liver; ELISpot, 50 spots/4 × 105 splenocytes). Data were mean ± SD, Student t-test. *P < 0.05; **P < 0.01; ***P < 0.001. ns Not significant. Source data are provided as a Source Data file
