Fig. 5
From: NKG2A is a NK cell exhaustion checkpoint for HCV persistence
HCV infection of hepatocytes impaired hepatic NK cells function. a Flowchart of NK functional inhibition assays. PHTs (2 × 105/mL) isolated from naive C/OTg mice were infected with HCVcc (MOI = 1) for 3 days before NPCs (1 × 106) were added. Yac-1 cells (1 × 105/well) or PMA (50 ng/mL) plus ionomycin (1 μM) were used to stimulate NK cells. b FACS analysis of intracellular IFN-γ and CD107a of NK cells 6 h after stimulation. c Indicated blocking antibodies and isotype IgG were added to the PHT/NK co-culture assay. IFN-γ expression of NK cells was detected 6 h after Yac-1 cell stimulation. d Measurement of intracellular IFN-γ in NK cells after supernatants of PHTs after HCV infection (HCV-CM) were added to NPCs. Yac-1 cells (1 × 105/well) or PMA (50 ng/mL) plus ionomycin (1 μM) were used to stimulate NK cells. e NPCs or MACS purified intrahepatic NK cells were co-cultured with or transwell separated from HCV-infected PHTs. IFN-γ expression of NK cells was detected 6 h after Yac-1 cell stimulation. Data were mean ± SD, Student t-test. *P < 0.05; **P < 0.01. ns Not significant. Source data are provided as a Source Data file
