Fig. 4

Loss of XPB NTD integrity induces an increase in KAT2A HAT activity. a rKAT2A and the recombinant HAT-ATAC module (rHAT-ATAC) containing KAT2A, ADA3, ADA2a, and SGF29 were resolved by SDS-PAGE followed by Coomassie staining. Core TFIIH containing p62, p52, p44, p34 and either XPB^WT (cIIH-XPB^WT) or XPB^F99S (cIIH-XPB^F99S) were resolved by SDS-PAGE followed by Coomassie staining. Source data are provided as a Source Data file. b One hundred nanograms of core TFIIH containing either XPB^WT (cIIH-XPB^WT) or XPB^F99S (cIIH-XPB^F99S) were incubated with 50 ng rKAT2A together with histone H3.3 and cold acetyl-CoA. Following resolution by SDS-PAGE, proteins were immunoblotted using polyclonal rabbit anti-H3, monoclonal mouse anti-H3K9Ac, mouse monoclonal anti-XPB, and polyclonal rabbit anti-KAT2A (SCBT) antibodies. Quantification of H3K9Ac was performed using ImageJ software and normalized with H3. Source data are provided as a Source Data file. c Twenty nanograms of rXPB^WT or rXPB^F99S were incubated with 50 ng of rKAT2A together with histone H3.3 and cold acetyl-CoA. Following incubation, reactions were treated as described in panel (b). Source data are provided as a Source Data file. d Twenty nanograms of rXPB^WT or rXPB^F99S were incubated with 200 ng of the rHAT-ATAC module together with histone H3.3 and cold Acetyl-CoA. Following incubation, reactions were treated as described in panel (b). Source data are provided as a Source Data file