Fig. 5
Higher-order chromatin decondensation induced by XPB mutants is due to KAT2A HAT activity. a U2OS17 cells were transfected either with siCTL or with siKAT2A for 72 h and cell extracts were resolved by SDS-PAGE and immunoblotted using polyclonal rabbit anti-KAT2A or polyclonal rabbit anti-tubulin antibodies. siCTL or siKAT2A transfected cells were re-transfected with either LacR-GFP, XPBWT-LacR-GFP, XPBF99S-LacR-GFP or XPB320–782-LacR-GFP for 24 h and the relative array volumes (Vol) were quantified as described above. Significant p-value are indicated (*** ≤ 0.001) and were obtained using a Kruskal Wallis test. Source data are provided as a Source Data file. b XP-B/CSF99S and XP-B/CSF99S + XPBWT cells were treated or not with MB-3 (200 μM) for 15 h and were subsequently labeled with polyclonal rabbit anti-tubulin and stained with DAPI. Cells were then reconstructed in 3D using Imaris Software (Bitplane) and the volumes of the cells and their nuclei were measured. The whisker box plot shows the volume of the nucleus/volume (Vol) of the cell (at least 30 cells for each condition, error bars represent SD from three independent experiments). The significant p-value is indicated (*** ≤ 0.001) and were obtained using a Kruskal Wallis test. Source data are provided as a Source Data file
