Fig. 6
KAT2A HAT activity induces inappropriate gene activation in XP-B/CSF99S cells. a RNA-seq analysis scatter plots comparing XP-B/CSF99S vs XP-B/CSF99S + XPBWT transcription profiles. Points show significantly over- (bottom) or under-(top) represented mRNA in XP-B/CSF99S cells compared to XP-B/CSF99S + XPBWT. All data were evaluated with the DESeq2 R package. For a given gene, its value is the normalized gene expression value relative to the mean of all samples belonging to the same condition. b A Comparative analysis of RNA-seq data from XP-B/CSF99S and XP-B/CSF99S + XPBWT. The pie chart indicates the number of genes that are up- and downregulated in the XP-B/CSF99S vs XP-B/CSF99S + XPBWT. c Pre-mRNA levels (error bars represent SD from three independent experiments) of LRRC42, NETO1, RNF130, RARβ2, and CYP26 were analyzed in XP-B/CSF99S or XP-B/CSF99S + XPBWT cells treated with either DMSO or DRB, as indicated. Data represent the relative expression levels of the pre-mRNA vs. GAPDH mRNA. Source data are provided as a Source Data file. d Pre-mRNA levels (error bars represent SD from three independent experiments) of LRRC42, NETO1, RNF130, RARβ2, and CYP26 were analyzed in XP-B/CSF99S or XP-B/CSF99S + XPBWT cells treated either with DMSO or with MB-3 (200 μM) as indicated. Data represent the relative expression levels of the pre-mRNA vs. GAPDH mRNA. Source data are provided as a Source Data file. e Pre-mRNA levels (error bars represent SD from three independent experiments) of LRRC42, NETO1, RNF130, RARβ2, and CYP26 were analyzed in XP-B/CSF99S or XP-B/CSF99S + XPBWT cells treated with either siCTL or siKAT2A, as indicated. Data represent the relative expression levels of the pre-mRNA vs. GAPDH mRNA. Source data are provided as a Source Data file. f Pre-mRNA levels (error bars represent SD from three independent experiments) of CYP26 and LRRC42 were analyzed in XP-B/CSF99S and XP-B/CSF99S + XPBWT cells as well as on stable clone 14 and clone 5 that were selected after transfection of cDNA coding for XPBT119P. Clone 14 does not express the transgene while clone 5 expresses only the transgene. Data represent the relative expression levels of the pre-mRNA vs. GAPDH mRNA. Source data are provided as a Source Data file
