Fig. 7
Chromatin modification and RNA Pol II TIC formation at the promoters of overexpressed genes in XP-B/CS cells. a, b Schematic representations of the RARβ2 (a) and RNF130 (b) gene regions, including the upstream (Us) and promoter (Pr 1 and 2, respectively) regions that were amplified (see in facing half-arrowheads). c–j ChIPs monitoring occupancy by KAT2A (using a polyclonal rabbit anti-KAT2A antibody (EpiGentek) Supplementary Figure 9) (c, d), H3K9ac/H3 (using monoclonal mouse anti-H3K9Ac and polyclonal rabbit anti-H3 antibodies) (e, f), TFIIB (using a polyclonal rabbit anti-TFIIB antibody) (g, h) and Pol II pS5 (using a monoclonal rat anti-Pol II pS5 antibody) (i, j) of either the Us or the Pr regions of RARβ2 (c, e, g, i) or the Pr region of RNF130 (d, f, h, j) in XP-B/CSF99S and XP-B/CSF99S + XPBWT cells treated either with DMSO or with MB-3 (200 μM). k–n ChIPs monitoring occupancy by the TFIIH core subunits p62 (using polyclonal rabbit anti-p62 antibody) (k, l) and XPBF99S (using polyclonal rabbit anti-XPB antibody) (m, n) of the Us or Pr regions of RARβ2 (k–m) or of the Pr region of RNF130 (l–n) in XP-B/CSF99S treated with either DMSO or MB-3
