Fig. 2
From: Chiral DNA sequences as commutable controls for clinical genomics
Matched performance of chiral DNA sequence pairs during next-generation sequencing. a Normalized sequencing coverage within synthetic chiral DNA sequence pairs (n = 8). Coverage profiles for human (fwd; blue and navy) and chiral (rev; red and orange) sequences are shown for two replicate NGS experiments. b Density scatter plots show the concordance of per-base coverage profiles between paired human and chiral (fwd/rev) sequences (left). For comparison, the concordance of identical human (fwd/fwd) sequences between replicate experiments is also shown (right). c Mismatch and indel sequencing error frequency profiles within one chiral pair of DNA sequences. d, e Scatter plots show concordance of mismatch (d) and indel (e) sequencing error frequency profiles between synthetic human and chiral (fwd/rev) DNA sequence pairs (left). For comparison, the concordance of identical human (fwd/fwd) sequences between replicate experiments is also shown (right)
