Fig. 3
From: Chiral DNA sequences as commutable controls for clinical genomics
Matched performance of chiral DNA sequence pairs during target-enriched NGS. a Schematic shows hybridization of a human DNA sequence to a labeled oligonucleotide probe (left) and hybridization of its chiral partner sequence to a mirrored oligonucleotide probe (right). This illustrates how human/chiral sequence pairs are captured via mirrored hybridization interactions. b Genome browser view shows alignments from duplicate target-enriched NGS experiments analyzing a human DNA sample (blue, navy) with synthetic chiral sequences (red, orange) added. Targeted genome regions and sequence features are indicated below. c Density scatter plots show the concordance of per-base coverage profiles for paired human/chiral regions (upper). For comparison, the concordance of identical human/human sequences (middle) and unpaired human/human sequences (lower) between replicate experiments are also shown
