Fig. 3

Hsp90 ATPase stimulation by Aha1p and Hch1p. a Schematic of Aha1p constructs harboring N and C terminal 6xHis-tags. b Stimulation of the Hsp82p ATPase activity by increasing concentrations of Aha1p (N terminal 6xHis-tag—blue diamonds; C terminal 6xHis-tag—black circles) and Aha1p^Δ11 (N terminal 6xHis-tag—green triangles; C terminal 6xHis-tag—purple squares). Reactions contained 1 μM Hsp82p and indicated concentration of co-chaperone (n = 3). c Stimulation of the Hsp82p ATPase activity by increasing concentrations of Hch1p (black circles) and Hch1p^Δ11 (purple squares). Reactions contained 4 μM Hsp82p and indicated concentration of co-chaperone (n = 3). d Stimulation of the Hsp82p ATPase activity by increasing concentrations of Aha1p^N (black circles) and Aha1p^N-Δ11 (green squares). Reactions contained 4 μM Hsp82p and indicated concentration of co-chaperone (n = 3). e Stimulation of the Sti1p-inhibited Hsp82p ATPase activity by increasing concentrations of Aha1p (black circles) and Aha1p^Δ11 (blue squares) in the presence of Cpr6p. Reactions contained 1 μM Hsp82p, 4 μM Sti1p, and 4 μM Cpr6p (n = 3). Error bars show standard error of the mean