Fig. 1 | Nature Communications

Fig. 1

From: The Arabidopsis H3K27me3 demethylase JUMONJI 13 is a temperature and photoperiod dependent flowering repressor

Fig. 1

JMJ13 is an H3K27me3 demethylase in vivo. a Schematic representation of GFP-tagged JMJ13 and JMJ13-H293A-E295A-GFP constructs. HD, helical domain. b, c Over-expression of JMJ13-GFP reduces the levels of H3K27me3 but not H3K27me2 and H3K27me1 in vivo. d, e Over-expression of JMJ13-H293A-E295A-GFP has no effect on H3K27 methylation. In b, d, the white arrows point to the transfected nuclei stained by methylation-specific histone antibodies (red, right panels), DAPI (blue, left panels), and the GFP signal from the JMJ13-GFP or JMJ13-H293A-E295A-GFP (green, middle panels), respectively. Scale bars, 2 μm. In c, e, more than 20 pairs of transfected nuclei versus non-transfected nuclei in the same field of view were observed and quantifications statistical analyzed. Error bars represent mean ± SE. Student’s t test was used to calculate the P value between JMJ13-GFP and WT. ****P value < 0.0001. The dots denote the individual data points. Source data are provided as a Source Data file.

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