Fig. 5
JMJ13 negatively modulates flowering time in a temperature- and photoperiod-dependent manner. a Three complementary lines Comp −1, −2 and −3 (pJMJ13:JMJ13-HAjmj13) showed similar flowering time with wild type in 22 °C LD conditions, but the complementary lines Comp-Mu −1, −2 and −3 with site mutation in the two conserved iron-binding amino acids (pJMJ13:JMJ13mu-HAjmj13), the complementary lines with truncation in JmjC Comp-ΔJmjc −1,−2, −3 (pJMJ13:JMJ13-ΔJmjc-HAjmj13) and zinc finger domain Comp-ΔZF −1, −2, −3 (pJMJ13:JMJ13-ΔZF-HAjmj13) remain early flowering. The plant images were created by the authors in this research. b The flowering time of complementary lines (Comp −1, −2, −3); (Comp-Mu −1, −2, −3); (Comp-ΔJmjc −1, −2, −3); (Comp-ΔZF 1, −2, −3) were assessed by counting rosette leaf numbers in bolting seedlings grown under 22 °C, 28 °C LD, and 22 °C, 28 °C SD conditions. Values are means ± SE of indicated number of plants for three independent biological repeats. N number was marked in the bottom of the column. Student’s t tests was used to calculate the P value between Col and other lines. ****P value < 0.0001; ***P < 0.001; **P < 0.01; *P value < 0.05; Bar = 1 cm. The individual data points are shown as dots. Source data are provided as a Source Data file
