Fig. 1 | Nature Communications

Fig. 1

From: Classification of the human phox homology (PX) domains based on their phosphoinositide binding specificities

Fig. 1

Membrane binding of the human PX domains by liposome pelleting assay. Bacterially expressed and purified PX domains with GST tags removed were incubated with artificial liposomes as indicated. Liposomes included POPC/POPE (PC/PE) as a negative control, Folch I as an indicator of broad membrane-binding activity, or PC/PE liposomes containing the specific lipids PtdIns3P, PtdIns4P, PtdIns5P, PtdIns(3,4)P2, PtdIns(3,5)P2, PtdIns(4,5)P2, PtdIns(3,4,5)P2, or PS. Samples were subjected to ultracentrifugation followed by SDS-PAGE and Coomassie staining of the unbound supernatant (S) and bound pellet (P) fractions. Gel filtration profiles of the purified proteins are shown in Supplementary Figure 1. SH3PXD2A (indicated by *) is the only protein that has its GST-tag intact

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