Fig. 5 | Nature Communications

Fig. 5

From: Classification of the human phox homology (PX) domains based on their phosphoinositide binding specificities

Fig. 5

Binding of SNX25 to phosphoinositides by NMR. a Plot of the average chemical shift changes in the 2D 1H-15N-HSQC spectra of the SNX25 PX domain upon addition of the indicated phosphoinositides. The SNX25 PX domain is shown in ribbon representation, with residues showing the greatest chemical shift changes The residues displaying significant chemical-shift changes on addition of the indicated phosphoinositides colored in red (∆δ > 0.1 ppm; ∆δ = [(0.17∆NH)2 + (∆HN)2]1/2)45. b Liposome pelleting assay for GST-SNX25 PX domain and mutants. GST-tagged PX domains were incubated with the indicated artificial liposomes, subject to ultracentrifugation, and the pellet ‘P’ and supernatant ‘S’ fractions analyzed by SDS-PAGE and Coomassie staining

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