Fig. 7 | Nature Communications

Fig. 7

From: Wnt1 silences chemokine genes in dendritic cells and induces adaptive immune resistance in lung adenocarcinoma

Fig. 7

Wnt1 silencing attenuates Wnt pathway activation in human LUAD cDCs. a Human CD1−cDCs were sorted from primary lung adenocarcinomas and paired juxta-tumor healthy lung and analyzed by RNAseq. Gene Set Enrichment Analysis (GSEA) enrichment plot (n = 4 per group). b Primary human lung adenocarcinomas were dispersed and total cells cultured in the presence of Wnt1-silencing RNA (siWnt1) or control siRNA loaded nanoparticles (Up). Wnt1 silencing was confirmed by western blot (bottom). Representative histograms showing active b-catenin in cDCs (up) and extracellular CD107 in CD8+ T cells (bottom). Data are representative of 3 independent experiments. Source data are provided as a Source Data file. Sequencing data are available with the accession code GSE124199

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