Fig. 4
YAPS6A overexpression impairs differentiation of β cells and promotes proliferation. a Experimental workflow for the lentiviral overexpression of a stabilized form of YAP (YAPS6A) during β-cell differentiation. b, c Flow cytometry of YAP expression and quantification of YAP + cells in LacZ control and YAP-overexpressing stem cell-derived cells during β-cell differentiation (assayed at stage 6, day 14). d–f Flow cytometry analysis and quantification of C-peptide and NKX6.1 coexpression in LacZ and YAPS6A-overexpressing cells at the end of stage 6 differentiation (stage 6, day 14). g–j Flow cytometry analysis and quantification of C-peptide and Ki67 expression in LacZ and YAPS6A-overexpressing cells. k–n Quantification of EdU staining (k, l) and cleaved Caspase-3 staining (m-n) in monohormonal C-peptide + and C-peptide- cells by flow cytometry at the end of stage 6 differentiation (stage 6, day 14). Data represent mean (center line) ± min to max (bounds of box), **p < 0.01, ***p < 0.001, ****p < 0.0001, two-sided student’s t test (n = 3 biologically independent samples per group)
