Fig. 1

Study design for benchmarking omics computational tools. to evaluate the accuracy of benchmarked computational tools, results obtained by running the computational tools are compared against the gold standard data (ground truth). First, biological samples are probed by regular measurement protocols (processes that generate omics data) (a). Raw omics data generated by these protocols serve as the input for examined computational tools (b, c). Results obtained by running computational tools are the final output of the omics pipeline (d). Gold standard data are produced by the benchmarking procedure and are based on technological protocol, expert manual evaluation, synthetic mock community, curated databases, or computational simulation (e). (Types of technologies available for use in the preparation of gold standard data are described in the section Preparation of Gold Standard Data.) Some of the techniques used to generate the gold standard data produce raw data, which needs to be analyzed (f); other techniques directly produce the gold standard data (g). Gold standard data obtained by or in conjunction with the raw omics data generated by regular measurement protocols enables researchers to use statistical metrics (h) and performance metrics to assess the computational cost and speed required to run the benchmarked computational tools (h), allowing the researcher to draw explicit, standardized comparison of existing computational algorithms. Methods with the best performances are located on the Pareto frontier and are identified as Pareto-efficient methods (i). A method is considered to be Pareto efficient if no other benchmarked method improves the score of one evaluation metric without degrading the score of another evaluation metric. (Evaluation methods and criteria for selecting the methods with the best performances are described in the section Selecting a Method with the Best Performance).