Fig. 9
From: USP32 regulates late endosomal transport and recycling through deubiquitylation of Rab7
Model of USP32 function in Rab7-mediated transport and recycling. a In the presence of USP32: non-ubiquitylated Rab7 can efficiently mediate minus end-directed transport, while C-terminal ubiquitination of Rab7 enables the switch to other functions, such as recycling from the late endosome/multi-vesicular body (LE/MVB). In turn, deubiquitylation of Rab7 by USP32 promotes fission of recycling tubules from the mother endosomes and release of Rab7 from the membrane (*refers to GDI (GDP Dissociation Inhibitor) associated with Rab7 upon release). b In the absence of USP32: lack of Rab7 deubiquitylation results in failure to resolve recycling tubules and inhibits liberation of Rab7 for subsequent functional cycles. (−): microtubule minus end, leading towards the perinuclear region; (+): microtubule plus end, leading towards the cell periphery
