Table 1 Heavy peptide standard sequences for quantification of phosphorylated and non-phosphorylated ERK1 and ERK2 by mass spectrometry

Species^a	Peptide^b	m/z	charge	t start [min]	t stop [min]	Max injection time [ms]	AGC target
ERK1_L_NP	GQPFDVGPR	486.7485	2	0	19	250	100,000
ERK1_H_NP	GQPFDVGPR_H	491.7527	2	0	19	250	100,000
ERK2_L_NP	GQVFDVGPR	487.7563	2	0	19	250	100,000
ERK2_H_NP	GQVFDVGPR_H	492.7605	2	0	19	250	100,000
ERK1_L_P	IADPEHDHTGFLpTEpYVATR	777.9943	3	19	32	500	50,000
ERK1_H_P	IADPEHDHTGFLpTEpYVATR_H	781.3304	3	19	32	100	50,000
ERK2_L_P	VADPDHDHTGFLpTEpYVATR	768.6505	3	19	32	500	50,000
ERK2_H_P	VADPDHDHTGFLpTEpYVATR_H	771.9866	3	19	32	100	50,000

AGC, automatic gain control
^a Peptide features: L light, H heavy, NP non-phospho, P phospho
^b Peptide sequences: pT phospho-threonine, pY phospho-tyrosine, R_H heavy isotope arginine

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