Fig. 6
From: Cortical recruitment determines learning dynamics and strategy
Discrimination training of multi-spot optogenetic patterns reveals a choice of learning strategy depending on the level of cortical recruitment. a Schematics of the optical setup to project arbitrary 2D light patterns onto the surface of auditory cortex. Blue light patterns from a Digital Micromirror Device (DMD) are collimated and deflected through the objective lens by a beam splitter. The surface of the cranial window can be simultaneously imaged by a CCD camera using external LEDs (green for blood vessel, red for intrinsic imaging). b Examples of light patterns used for the discrimination task. Scale bars: 600 μm. c Tonotopic maps obtained with intrinsic imaging for two mice, and localization of the three optogenetic stimulation spots in the same high, low, and mid frequency fields. d Four example learning curves for the optogenetic discrimination task. Gray: smaller Ŝ−; color: larger Ŝ−. e (left) Light pattern in the task in which the S− stimulus has higher level of cortical recruitment (Ŝ− >Ŝ+). Ĉ = common component of S+ and S− stimuli. (right) Response probability for the two learnt target stimuli (Ĉ & Ŝ+vs Ĉ & Ŝ−), for the presentation of the common part of the stimuli alone (Ĉ, yellow), for the specific parts of the stimuli alone (Ŝ+; Ŝ−) and in absence of stimulation (blank). Ten to 15 catch trials per mouse. Mean and individual data points, n = 6 mice (except Ŝ+ & Ŝ−, n = 2 mice). Gray dots: single animals. f Same as e, but with Ŝ+ larger than Ŝ−. Mean and individual data points, n = 7 mice (except Ŝ+ & Ŝ−, n = 3 mice). Scale bars: 600 μm. Source data are provided as a Source Data file
