Fig. 7

The 25HC-integrin-FAK signaling network regulates proinflammatory response during virus infection. a 25HC levels in the medium supernatant of BMDMs infected with human respiratory syncytial virus (RSV). b TNF production from wild-type (WT) and C25H knockout (KO) BMDMs infected with RSV. c Western blot and densitometric analyses of FAK activation (phospho-FAK, Tyr925) status in RSV-infected BMDMs. d Western blot and densitometric analyses of FAK activation (phospho-FAK, Tyr925) status in influenza A virus (IAV)-infected BMDMs. e IL-6 production from RSV-infected WT and FAK KO MEFs. f IL-6 production from IAV-infected WT and FAK KO MEFs. g TNF production from RSV-infected BMDMs in the presence of either control IgG or α5β1 integrin blocking antibody (Ab). h IL-6 production from IAV-infected BMDMs in the presence of either control IgG or α5β1 integrin blocking antibody. i TNF secretion from IAV-infected WT and β3 integrin-deficient (β3^+/− cells) BMDMs. j IL-6 secretion from IAV-infected THP-1 cells in the presence of either control IgG or αvβ3 integrin blocking antibody. k IL-6 in the lungs of mice injected (via intratracheal or I.T route) with 25HC (5 mg/kg; 6 h) in the presence of either IgG or β1 integrin blocking antibody (Ab) administered to the mice via I.T route (n = 4). l IL-6 in the lungs of mice injected (via I.T route) with 25HC (5 mg/kg; 6 h) in the presence of either vehicle (control) or FAK inhibitor (PND-1186) administered to the mice via I.T route (n = 4). The ELISA values (mean ± standard deviation) are representative from two or three independent experiments (n = 4). *p ≤ 0.05 using a Student’s t-test. The densitometric quantification values for phospho-FAK (p-FAK) immunoblot represent the ratio of phospho-FAK:actin and the fold-induction was calculated after normalizing with the control mock-infected group. The densitometric values represent the mean ± standard deviation from three independent studies. *p ≤ 0.05 using a Student’s t-test