Fig. 7

Induction of ADRN-to-MES reprogramming is tumorigenic in vivo. a Growth of SH-SY5Y-NOTCH3-IC xenograft with (+dox, red) or without (−dox, blue) doxycycline in the drinking water. Error bars represent standard deviation. Y-axis shows relative tumor growth; x-axis depicts time in days. Source data are provided as a Source Data file. b mRNA analysis of ADRN and MES signature scores of SH-SY5Y-NOTCH3-IC xenograft tumors with induction of NOTCH3-IC. Non-induced control tumors (n = 4) are depicted in blue, tumors with 7 or 14 days NOTCH3-IC expression (n = 4) are shown in red and purple, respectively. Dashed lines group various neuroblastoma cell lines (gray dots) according to MES and ADRN phenotypes for reference (see ref. ¹). c Immunohistochemistry of NCAM1 and MAML2 on tumors with (+dox) or without (−dox) 7 days of NOTCH3-IC induction. dox, doxycycline. Scale bar, 100 µM