Fig. 2
Development of heterologous neutralization by the mature BF520.1 lineage. a–c Abilities of BF520.1 inferred heavy chain (a), light chain (b), and paired (c) lineage intermediates to neutralize a multi-clade and multi-tier panel of 13 viruses, all of which are neutralized by mature BF520.1. Heavy- and light-chain pairings are indicated along the top row (black text) along with progressive amino acid substitutions (red text). Schematics depict antibody lineage pairings with darker red shades indicating greater maturity. Breadth percentages refer to neutralization of viruses within the 13-virus panel with IC50 values of < 20 µg ml−1; darker blue shades indicate increased breadth. SHM percentages refer to nucleotide mutation. Geometric mean IC50 values (in µg ml−1) refer to Tier 2 viruses only. d, e Amino acid alignment of BF520.1 naive, Bayesian (0–6 months pi) and rationally inferred (6–12 months pi) lineage intermediates, mature, and minimally mutated heavy (d) and light (e) chain sequences
