Fig. 3
Contribution of kappa chain maturation to HIV binding. a, b BLI representative reference-subtracted sensorgrams for each interaction between the BG505.SOSIP.664 (ligand) and BF520.1 naiveVHmatureVK (a) and matureVHnaiveVK (b) (analytes). IgG concentrations ranged from 667 to 42 nM. The gray lines show 0 μM IgG. KD, Kon, and Kdis are shown from best fitting (green lines) to a 1:2 bivalent analyte model of binding
