Table 1 Illumina Miseq library preparation primers
Conc. (nM) | Primer ID | Sequence |
|---|---|---|
| Â | RT-PCR | Â |
500 | 3′IgM (OUTER) | CCACTTCGTTTGTATCCAACG |
500 | 3′IgG (OUTER) | GCCGGGAAGGTGTGCACGCCGCTGGTC |
500 | 3′IgK (OUTER) | GTCCTGCTCTGTGACACTCTC |
500 | 3′IgL (OUTER) | TGTTGCTCTGTTTGGAGGG |
| Â | PCR 1 | Â |
800 | 3′IgM (INNER) | GCATTCTCACAGGAGACGAGG |
800 | 3′IgG (INNER) | CCGGTTCAGGGAAGTAGTCCTTGAC |
800 | 3′IgK (INNER) | ATTCAGCAGGCACACAACAGAGGC |
800 | 3′IgL (INNER) | AGACACACTAGTGTGGCCTTG |
800 | vv535: Step out primer | GACAAGCAGTGGTATCAACGCAG |
| Â | PCR 2 | Â |
400 | MiSeq HsRh IgM CH1 R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGGTTGGGGCGGATGCACT |
400 | MiSeq HsRh IgG CH1 R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGGGGAAGACCGATGGGCCCTT |
400 | MiSeq HsRh IgK CL1 R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGAAGACAGATGGTGCAGCC |
400 | MiSeq HsRh IgL CL1 R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGAACAGAGTGACCGTGGG |
400 | vv539: MiSeq-adapted step out primer | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCACTCTATCCGACAAGCAGTGGTATC |
