Table 2 Cryo-EM data collection, refinement parameters and model statistics

Magnification

130,000

Voltage (kV)

300

Electron exposure (e–/Ų)

66

Defocus range (μm)

1.0–3.5

Pixel size (Å)

0.55

Symmetry imposed

C3

Initial particle images (no.)

559,022

Final particle images (no.)

118,972

Map resolution (Å)

4.8

   FSC threshold

0.143

Map resolution range (Å)

4.53–7.52

Refinement

 Initial model used (PDB code)

–

 Model resolution (Å)

9.02

    FSC threshold

0.143

 Model resolution range (Å)

9.02

 Map sharpening B factor (Ų)

−179.793

 Model composition

    Non-hydrogen atoms

21,447

    Protein residues

2595

    Ligands

192

R.m.s. deviations

    Bond lengths (Å)

0.85

    Bond angles (°)

0.96

Validation

    Clashscore

35

    Poor rotamers (%)

1

Ramachandran plot

    Favored (%)

89

    Allowed (%)

9

    Disallowed (%)

2