Fig. 3
From: The lncRNA Neat1 promotes activation of inflammasomes in macrophages
The effect of Neat1 on the NLRP3 inflammasome activation is independent of IL-6. a BMDMs derived from IL-6 wild-type (IL-6+/+) or knockout (IL-6−/−) mice were primed with LPS and then treated with nigericin. Protein levels in total cell lysates and supernatant fraction were analyzed by western blotting. b–d IL-6+/+ or IL-6−/− BMDMs were transfected with control siRNA (b–d), Neat1 siRNA #1 (b), Neat1 siRNA #2 (c), or Neat1_2 siRNA (d). Twenty-four hours later, cells were stimulated with LPS and nigericin. WCL and SN of above cells were subjected to western blotting with indicated antibodies. Source data are provided as a Source Data file
